フェンタニルによるμオピオイド受容体インターナリゼーションの検討
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概要
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Introduction: µ-opioid receptor (MOR) internalization is caused by (DAMGO) and etorphine, but not by morphine in vitro and in vivo. MOR internalization caused by fentanyl is demonstrated only in vitro. The relationship between MOR internalization and analgesic effect caused by fentanyl has not been studied. In addition, the role of MOR internalization caused by opioid agonists in the analgesic effect in vivo has not been well established. In the present study, therefore, we examined whether fentanyl causes MOR internalization in vivo or not and also studied the relationship between MOR internalization and analgesic effect of opioid agonists. Methods: The protocol was approved by our animal research and use committee. Male Sprague-Dawley rats weighing 300–330 g were implanted with intrathecal catheters at the level of L4–5. Tail flick test was performed at 30 min after intrathecal administration of morphine, DAMGO, fentanyl, or saline. Immediately after the test, rats were perfused and the spinal cord was removed. MOR distribution was assessed by immunohistochemical staining of MOR in the dorsal horn neurons. Results: The opioid agonists exerted analgesic effect assessed by tail flick test at 30 min after injection. In morphine treated rats, no MOR internalization was observed in the laminae I and II neurons of the spinal cord, as was seen with saline. DAMGO caused MOR internalization in almost all of the neurons expressing MOR. Though fentanyl also produced internalization in the laminae I and II neurons, the internalization was observed in approximately half of the neurons expressing MOR, and the distribution of internalized MOR was different from that induced by DAMGO. Conclusion: Intrathecally administered fentanyl caused MOR internalization in the rat spinal dorsal horn neurons. MOR distribution showed different patterns depending on opioid agonists used. These results suggest that the MOR internalization does not seem to be involved in analgesic effect produced by opioid agonist.
- 日本疼痛学会の論文
日本疼痛学会 | 論文
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