Study of the Potentiality of a Cell Culture Medium of Autologous Blood Serum for Cell Transplantation Therapy for Injured Vocal Folds
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概要
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We succeeded in the regeneration of an injured vocal fold in a canine by the transplantation of bone marrow derived stromal cells (BSCs). This therapy required a cell culture. The safety of the ordinal culture medium with an animal serum such as fetal bovine serum (FBS), however, has not yet been confirmed. In this <I>in vitro</I> study, we investigated the potentiality of a culture medium of autologous blood serum (ABS) which would be the safest additive for clinical applications.<BR>Canine ABS and the following three types of medium were prepared. Mediums I, II and III were alpha-Minimum Essential Medium (alpha-MEM) as blood serum free medium for control, alpha-MEM with 15% ABS and Dulbecco's Modified Eagle's Medium (D-MEM) with 10% FBS for ordinal medium, respectively. The growth rates of the canine cultured BSCs in these three mediums were estimated.<BR>After 2 weeks culture, the number of cells in medium I, II and III increased by 2, 8-10 and 14-15 times, respectively. These results showed that ABS had a satisfactory cell growth promoting action to apply clinically as a substitute for FBS.
- The Japan Laryngological Associationの論文
The Japan Laryngological Association | 論文
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