Analysis of the Cell Kinetics of Brain Tumors Using Flow Cytometry and Its Application to the Sensitivity Test to Antineoplastic Agents
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Flow cytometry (FCM) has been used widely for clinical diagnosis and evaluation of chemotherapy. The author has conducted monolayer-culture of brain tumor cells and reported the changes of DNA-histograms of brain tumor cells for antineoplastic agents. In this paper, a new application of FCM was established to evaluate the choice of the best antineoplastic agent in chemotherapy for malignat brain tumor.<BR>After adjusting cell preparation and eva luating the analytic system of FCM, the sensitivity test to antineoplastic agents was developed through preliminary studies using the established brain tumor cell line. Antineoplastic agents were challenged to the cells in monolayer-culture, and the changes of DNA-histograms as well as cell viability were analyzed by FCM. Cell viability was measured by the FDA (Fluorescein Diacetate) vital staining method, and propidium iodide was used for the analysis of DNA-histograms. Judgment of the best agent was conducted, based on its effects on the changes of their cell kinetics.<BR>Cytocydal and cytostatic effects were evaluated quantita tively, and the results showed a well coincidence with the sensitivity of the cells to the appropriate antineoplastic agent.<BR>Secondly, the above system was applied to the clinical cases of 15 malignant b rain tumors. The best antineoplastic agent was selected for each case through this method and good therapeutic effects were demonstrated. The present results suggest that the above method is of much clinical value.
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The Medical Society of Kansai Medical University | 論文
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