緑膿菌の色素産生に関する研究 特にメラニン株No.117の産生する褐色々素について
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概要
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A melanogenic strain of <I>Ps. aeruginosa</I>, No.117, was inoculated to King A liquid medium, using Polypeptone (Takeda) as carbon and nitrogen sources, and incubated at 37°C for over 1 month. The Brownish black precipitate, obtained from the culture filtrate by adding the 20% CuSO<SUB>4</SUB> solution, was dissolved in 1M NH<SUB>4</SUB>OH and fractionated by gel-filtration through the Sephadex G-25 column. Melanin I was obtanied by adding diluted acetic acid solution to Fraction I, and Melanin II was obtained by saturating Fr. II with methyl-ethyl keton.<BR>Melanin I is amorphous black substance, much soluble in alkaline solution and a little in HCl 5 N or more, and insoluble in various organic solvents. Analytical found: Melanin I; C 44.6, H 5.37, N 11.35, calcd. for (C<SUB>5</SUB>H<SUB>8</SUB>NO<SUB>2</SUB>)n; Melanin II; C 51.36, H 6.09, N 11.15, calcd. for (C<SUB>5</SUB>H<SUB>7</SUB>NO<SUB>2</SUB>)n. Spectrophotometric studies of Melanin I revealed no specific absorption.<BR>Hydrolysis of Melanin I revealed the presence of 13 amino acids which, by twodimensional chromatography, could not be distinguished from glycine, alanine, vialne, leucine, serine, phenylalanine, tyrosine, tryptophan, proline, arginine, histidine, glutamic acid and aspartic acid.<BR>The alkaline solution of the hydrolysis-residue was brownish and showed no electrophoretic mobility at pH 8.6. Both the Melanin I and its hydrolysis-residue, dissolved in alkaline solution, were faded by hydrogen peroxide and changed into three or more unknown fluorescent substances.
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