Experimental Studies on the Differentiation of Lens with Amphibian Larvae (Hyvobius nebulosus)
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Using Hynobius nebulosus larvae, a species of urodela, the rostral half of the optic cup or eye ball was extirpated. The lens or its primordium was left intact and after development of a certain period, the differentiation coditions and also the influences from the surrounding tissues were examined. The developmental stage at time of the operation was 26th stage (the earliest stage of eye cup) or 37th stage (the earliest stage of fore-limb bud) and the operated material was kept alive for 20-50 days for further development.<BR>Experimental results are as follows.<BR>1. In the operation of the 26 th stage the average volume of the operated eye ball against the healthy eye was 45-50%, while on the 37th stage it was 25-30%. The mean volume of the lens of the operated eye was respectively 40-50% and 33-37% when compared with the lens of not-operated eye. From these results, it is considered that the earlier the developmental stage (at time of operation) is, the better results is obtained in regeneration or differentiation.<BR>2. The volume of lens of the operated eye is of irregular size and does not correspond to the size of the operated eye ball; and also there is no correlation between the size of the ocular chamber and differentiation degree.<BR>3. The quantitative differentiation of the lens of the operated eye generally shows good results when its position to the surrounding tissues is close to normal, and have poor results when some extraordinary factors exits. On the above findings, it is considered that the retina or the eye cup determines the direction of differentiation of the lens and also inducts its promotion. The surrounding tissues (such as iris, cornea etc. ) when in its normal positions are also determining factors.<BR>4. The lens of the operated eye, in all cases, show good qualitative results of differentiation. Especially when the operation is conducted on the 26th stage, since the stage is so early and the lens primordium is not formed yet, the affects of the operation are nearly none. In case of the operation on the 37th stage, since the differentiation is advanced considerably and the lens has taken its form already, the affects of the operation cannot be determined.<BR>5. In the operated eye, these is no correlation between the dimensions and thickness of epidermis on the cornea area and differentiation degree of the lens.<BR>6. The differentiation degree of the lens in the operated eye has the tendency to increase relatively, if the restoration process of the retina after the operation nears normalcy, and when the arrangement of the respective layers of the retina are in perfect order.
- The Medical Society of Kansai Medical Universityの論文
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