Partial Purification and Characterization of α-Glucosidase from Rothia dentocariosa

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Disaccharidases of oral bacteria, especially α-glucosidase and β-fructofuranosidase, are considered to play an important role in the induction of dental caries. Upon the examination of disaccharidases from several strains of saccharolytic cral bacteria, we found all of those bacteria to be capable of hydrolyzing the glycosidic linkage of sucrose. One species of bacteria, Rothia dentocariosa, was found to contain a single disaccharidase, α-glucosidase. This enzyme was partially purified by ammonium sulfate precipitation, gel filtration and ion-exchange column chromatography. The optimum pH and temperature for the enzyme activity was found to be 6.8-7.0 and 40°C, respectively. The enzyme activity was strongly inhibited by Ag+, Hg2+, Cu2+, Fe2+ and Tris (Hydroximethyl) aminomethane.
昭和大学・昭和歯学会の論文