Differentiation of <I>Streptococcus sobrinus</I> and <I>Streptococcus downei</I> by DNA Hybridization
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概要
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<I>Streptococcus sobrinus</I> and <I>Streptococcus downei</I> are closely related cariogenic species. A DNA fragment (1.2 kb) of a central region of the <I>S. sobrinus</I> dextranase gene (dex) was amplified by polymerase chain reaction and utilized for a DNA probe to differentiate <I>S. sobrinus</I> and <I>S. downei</I>. The <I>dex</I> probe was dot-hybridized to the reference strains (17 strains of 5 species) of mutans streptococci and other related strains (13 strains of 9 species). The probe hybridized only to both <I>S. sobrinus</I> and <I>S. downei</I> at a highly stringent condition, but to none of the other mutans streptococci nor other gram-positive cocci. To differentiate <I>S. sobrinus</I> and <I>S. downei</I>, Southern blot hybridization was carried out against restriction fragments of chromosomal DNAs with the <I>dex</I> probe. The probe showed species-specific hybridization patterns to the <I>Eco</I>RI, <I>Eco</I>RV, <I>Hind</I>III and <I>Pvu</I>II-fragments. This result suggests that hybridization analysis with the <I>dex</I> DNA probe is useful for the detection and differentiation of <I>S. sobrinus</I> and <I>S. downei</I>.
- 昭和大学・昭和歯学会の論文
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