Optimum Media for Outdoor Large-scale and Indoor Small-scale Batch Style Culture of Nannochloropsis oculata

概要

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A new enriched seawater medium for Nannochloropsis (ESM-NA medium) using analytical grade inorganic salts for indoor small-scale culture of Nannochloropsis oculata was formulated by removing ineffective chemicals as nutrients from Guillard F medium. In addition, a fertilized seawater medium for outdoor large-scale culture of the alga (FSM-NA medium) was devised in consideration of cost reduction. The composition of ESM-NA medium was NaNO3; 4.41 mM, NaH2PO4 • H2O; 90μM, Fe-EDTA; 29.7μM, MnCl2 • 4H2O; 2.2μM, ZnSO4 • 7H2O; 0.38μM and CoCl2 • 6H2O; 0.126μM, while that of FSM-NA medium was KNO3; 5.3 mM, NH4H2PO4; 0.66 mM, Clewat 32; 20 mg/l and Clewat Fe; 3 mg/l. The growth rate of N. oculata in FSM-NA medium was higher than that in YASHIMA medium which has been commonly used in Japanese hatcheries. Moreover, it can be considered that the contamination of FSM-NA medium into larval rearing water at the same time as the supplementation of N. oculata was little effect to the survival and the growth of fish larvae. Therefore, FSM-NA and ESM-NA media seem to be optimal for outdoor large-scale and indoor small-scale culture of N. oculata, respectively.
日本水産増殖学会の論文