Storage of Plant Cell by Using Xenon Hydrate
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概要
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Xe hydrate formation was applied to the plant cell for the basis of storage of agricultural produce. Firstly, the formation condition of Xe hydrate crystal in ultra pure water and crystal size distribution were examined. For the formation of Xe hydrate, the conditions were examined at the temperature of 1 and 5℃ and the Xe pressure from 0.7 to 1.0MPa. The smallest size of hydrate crystals formed at the condition of 1℃and 1.0MPa of Xe pressure. Secondly, Xe hydrate formation in barley coleoptile cells was confirmed experimentally and the viable ratio of cells after 12h storage was determined by applying the condition of 1 ℃ and 1.0MPa of Xe pressure. In addition, barley coleoptile cells were stored at -20 ℃ for 12h and viable ratio of cells was examined. The viable ratio of cells stored by the formation of Xe hydrate at each storage period was higher than that stored by the formation of ice crystals at -20℃. One reason of this observation could be a partial formation of Xe hydrate in cells. The other reason could be a smaller size of Xe hydrate resulting in less damage than ice crystal.
- 公益社団法人 日本冷凍空調学会の論文
公益社団法人 日本冷凍空調学会 | 論文
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