MACROPHAGE RESPONSIVENESS TO LIGHT THERAPY - A DOSE RESPONSE STUDY
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概要
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Macrophage-like U-937 cells were exposed in vitro to noncoherent 660 nm wavelength light produced by a superluminous diode at a power density of 120 mW/cm2 over a range of energy densities from 2.4 - 9.6 J/cm2. Twelve hours after irradiation, the medium in which the macrophages had been growing and which contained growth factors secreted by them was added to 3T3 fibroblasts, also growing in vitro, and its effect on fibroblast proliferation assessed. It was found that by 96 h after addition of the macrophage-conditioned medium there was a statistically significant increase in fibroblast proliferation in the cells exposed to supernatants from the experimental compared with the sham-irradiated macrophages. The most effective energy density was found to be 7.2 J/cm2 and the least effective 9.6 J/cm2.
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