イネいもち病菌株Ｋｙｕ９４３９０１３およびＯＳ９９‐Ｇ‐７ａを個体識別するＤＮＡマーカー
スポンサーリンク
概要
- 論文の詳細を見る
By using a primer set of pJF1 and pJR1, polymerase chain reaction (PCR) fragments, ca. 1,300 bp and ca. 2,700 bp were amplified from <I>Pyricularia grisea</I> isolates, Kyu9439013 and OS99-G-7a, respectively. The fragments were found to be unique to each isolate, suggesting that these could be used as specific DNA markers for the 2 isolates. Further investigation confirmed the specificity of the fragments: in the 480 isolates examined, some showed a fragment of ca. 800 bp, while others showed none. The specific markers of the 2 isolates were stably detected after 50 times transfer on medium and 7 times infection on rice leaves. Further, virulence of the 2 isolates was not reduced after the rice infection. Thus, since the isolates Kyu9439013 and OS99-G7a can be clearly identified by a specific DNA marker, these can be used as efficient tools to trace the rice blast pathogen in natural fields.
- 九州病害虫研究会の論文
九州病害虫研究会 | 論文
- コムギ赤かび病防除薬剤の耐雨性の評価(2)散布から降雨開始までの時間と液剤の効果の関係および降雨強度が粉剤の効果に及ぼす影響
- 3種アザミウマ類に対するアカメガシワクダアザミウマの捕食能力
- 果菜類におけるアカメガシワクダアザミウマの産卵および生息部位
- 鹿児島県内の花きほ場で発生する主要アザミウマ類3種に対する各種薬剤の殺虫効果
- アカメガシワクダアザミウマの発育と繁殖に必要なスジコナマダラメイガ卵量