河川付着性珪藻を用いた生長阻害試験方法の開発
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概要
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A growth inhibition test method using fresh water attached diatoms was developed by improving microplate algal assay technique for planktonic green algae. Applying the solid culture technique for the pre-culture, using the flat bottom microplate for the test vessel and shaking the microplate at least twice a day were necessary procedures for ensuring the reproducibility of the test using attached diatoms.<BR> Reproducible result was obtained from seven repetitions of growth inhibition tests using dimethametryn, a triazine herbicide on Achnanthidium minutissimum of attached diatom. The average ErC<SUB>50</SUB> values calculated from these tests was 7.9μg/l (<I>n</I>=7, SE=1.3). <BR> The optimum culture condition and the adaptability as test organism were evaluated in ten kind of attached diatoms (<I>A. minutissimum, Craticula molestiformis, Eolimna minima, Eolimna subminuscula, Fistulifera saprophila, Mayamaea atomus, Nitzschia palea, Planothidium frequentissimum, Planothidium lanceolatum, Sellaphora seminulum</I>). <BR> The optimum culture temperature was 15-30°C. The difference of illumination intensity (c.a.2,000lux or c.a.5,000lux) did not influence the growth rate of every diatom at the optimum culture temperature. It is concluded that <I>C. molestiformis, E. minima, E. subminuscula, F. saprophila, M. atomus</I> and <I>N. palea</I> are suitable test species in these diatoms in terms of high growth potential and that <I>M. atomus</I> and <I>N. palea</I> are valuable test species in terms of habitat diversity and easiness of isolation.
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