カワウ野生個体と培養細胞における遺伝子発現プロファイルの比較
スポンサーリンク
概要
- 論文の詳細を見る
We have analyzed the gene expression profiles in the liver of wild common cormorants (<I>Phalacrocorax carbo</I>) using microarray platform, and have predicted the potential toxic effects of persistent organic pollutants (POPs) and emerging POPs. However, when applying microarray technology to wild animals, it is quite difficult to clarify the cause-andeffect relationship between accumulated contaminants and gene expression alterations. Therefore, in the present study, we isolated liver cells from wild cormorant embryos, and the cells were cultured and treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or 3,3′,4,4′,5-pentachlorobiphenyl (PCB126). The gene expression profiles in the cultured cells were monitored by microarray analysis, and responses to dioxins were then compared between livers (<I>in vivo</I>) and cultured cells (<I>in vitro</I>) from wild cormorants. The cultured liver cells clearly exhibited responses to the exposure of TCDD or PCB126 including inductions of cytochrome P450 1A genes. Whereas fifteen genes on the microarray showed similar effects between in vivo and in vitro tests, indicating that these genes might be affected directly by dioxin exposure, the responses of other genes were different between wild cormorant livers and cultured cells. Therefore, we compared the 'biological process' gene ontology (GO) terms of dioxin-responsive genes between <I>in vivo</I> and <I>in vitro</I> tests. The most of annotated GO terms were shared in the livers and cultured cells, implying that the effects by dioxins were similar between both groups. Thus, gene expression profiling in the cells isolated from cormorants might be useful for evaluating chemical effects on the wild population.
- 日本環境毒性学会の論文
日本環境毒性学会 | 論文
- ミジンコ致死試験による農業地帯を流下する河川水の毒性評価
- バイカルアザラシCYP1A1/1A2 cDNAの同定およびダイオキシン類蓄積濃度と各mRNA発現量の関係 (第10回バイオアッセイ研究会・日本環境毒性学会合同発表会奨励賞授賞研究要旨)
- 山梨県内の河川に生息するコイの肝膵臓中メタロチオネイン量
- 環境汚染バイオマーカーとしてのメタロチオネインの有用性 (特集 環境影響評価のバイオマーカー)
- 酸素発生速度に基づく迅速な藻類毒性試験