水試料の効率的なｕｍｕ試験方法の開発
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概要
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In order to evaluate genotoxicity of water samples such as river water and tap water by the umu-test, improvement of ISO 13829 method and an efficient solid phase extractionconcentration method for pretreatment of water samples were studied. A quantitative measure of DNA-damaging potential, GA(Genotoxic Activity) was proposed to compare the strength of genotoxicity among various water samples. Then, temperature and duration of the first bacteria culture were modified from 37 °C, 8-12hr to 30 °C, 16-18hr to improve the performance of the ISO method, and the concentration of dimethylsulfoxide (DMSO) in the test solution was increased from 4.5% of the ISO method to 12% based on no growth inhibition of bacteria. As a result, sensitivity of the method is suggested to be improved 2.67 times compared with the ISO method. A high performance porous polystyrene resin, Sep-pak plus PS-2 cartridge, was used to enrich genotoxicants in water samples. By comparison with 7 other volatile organic solvents, DMSO was proved to be most effective to desorb genotoxicants from PS-2. One PS-2 cartridge was able to adsorb genotoxicants from 1.5<I>l</I> of water samples those DOC are under 10mg/<I>l</I>, and the adsorbed genotoxicants could be completely desorbed with 1.5 ml of DMSO. It was confirmed from the applications to various water samples, such as tap water, river water and treated sewage water that the genotoxicity could be evaluated quantitatively by the developed method.
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