Growth promotion of rice by inoculation of acid-tolerant, N2-fixing bacteria isolated from acid sulfate paddy soil in South Kalimantan, Indonesia
スポンサーリンク
概要
- 論文の詳細を見る
In South Kalimantan, Indonesia, a large area of rice paddy field is suffered with acid sulfate soil and the productivity of rice is supposed to be low. While in some paddy fields we have found that the yield of rice is larger than 5 ton ha-1 without any fertilizer. There assumed to be the contribution of nitrogen fixing bacteria for rice production by biological nitrogen fixation (BNF) under those low pH condition. We have screened bacteria from the rhizosphere and rhizoplane of local rice plant growing in natural field based on their growth on nitrogen free medium. We have obtained 26 isolates, and the majority of isolated bacteria belonged to the β-proteobacteria (34.6 %) and others are α-proteobacteria (19.2 %), γ-proteobacteria (0.38 %), Bacilli (19.2 %), and Actinobacteria (23.1 %) based on their 16S rDNA sequence. We have determined their nitrogenase activity and the existence of nifH gene by using PCR technique. Though all of the isolates can grow on nitrogen free medium, only five isolates belong to β-proteobacteria have high nitrogenase activity. The existence of nifH gene was also confirmed in theses five isolates by using PCR technique. We presume that one of the mechanisms of the adaptation of these bacteria under acid sulfate soil is changing the surrounding pH. In bacterial culture medium, the change of pH during the incubation of these isolates was deeply regulated by the existence of several organic acid anions. In rice culture with soil experiments, under initial soil pH 3.5 condition, though rice growth was inhibited with the addition of 500 μM Al, inoculation with obtained isolate significantly alleviated Al stress. It is suggested that beneficial interactions of rice and free living nitrogen fixing bacteria might be operating to reducing root Al stress in acid sulfate soil.
- 日本熱帯生態学会の論文
日本熱帯生態学会 | 論文
- クロロプラストatpB-rbcL intergene の塩基配列に基づくアカネ科着生性アリ植物の分子系統学的研究
- マレーシア国サラワク州ランビル国立公園で発見されたリス媒
- マウスがん抗原--腫瘍特異的変異ペプチドと野生型ペプチド (特集 免疫系はがん細胞を拒絶できるか) -- (T細胞認識がん抗原をタ-ゲットとした免疫治療)
- サケラート環境研究所の乾性常緑林と乾性落葉林の土壌の性質の違い
- 転換期にあるカリマンタン「森林伐採フロンティア」から「土地開発フロンティア」へ