Anti-proliferative effects and induction of apoptosis by liquiritigenin in human hepatocellular carcinoma SMMC-7721 cells
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概要
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Liquiritigenin is a kind of flavonoid extracted from licorice. To study its anti-tumor effects and mechanisms, SMMC-7721 cells were treated with liquiritigenin at serial concentrations. MTT assay was used to examine the growth inhibitory effects of liquiritigenin. Apoptotic morphological were detected using Hoechst 33258 staining. The apoptosis rate was determined by flow cytometry. P53, bcl-2 and procaspase-3 were analyzed using the Western blot method. The activity of caspase-3 was determined using the caspase-3 activity kit. In the study, liquiritigenin for SMMC-7721 cells growth showed a strong inhibitory effect in a dose-and time-dependent manner. Apoptotic morphological changes in the nuclear chromatin of cells were observed, and flow cytometric analysis demonstrated that apoptosis rates in SMMC-7721 cells with liquiritigenin treatment were 7.48%～41.17%. Western blot results revealed that p53 expression level was increased in a dose-dependent manner after liquiritigenin treatment, and liquiritigenin (0.4 mM) increased the expression of p53 approximately 9-flod compared with control group. In contrast, the expression of bcl-2 protein was decreased after liquiritigenin treatment. The results also revealed that liquiritigenin significantly decreased the expression level of procaspase-3, whereas the activity of caspase-3 increased. The present study indicated that liquiritigenin, probably through the regulation of p53 and bcl-2, inhibited cell proliferation and induced apoptosis for SMMC-7721 cells followed by their down-stream molecular caspase-3 activation.
- 和漢医薬学会の論文
和漢医薬学会 | 論文
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