Kampo formulations and allergic inflammatory diseases:- Efficacy for murine IgE-mediated triphasic cutaneous reaction -
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We found that passive sensitization with anti-DNP IgE antibody followed by the challenge with DNFB to the mouse ear can induce the triphasic cutaneous reactions (ear swelling) of immediate phase response (IPR), late phase response (LPR) and very late phase response (vLPR), peaking at 1 h, 24 h and 8 days after the challenge, respectively. IPR was absent in mast cell-deficient mice but LPR was sufficiently observed, and vLPR was partly attenuated. LPR is a T cell-independent response, while vLPR is almost completely absent in T cell-deficient nude mice. Thus, the third phase response (vLPR) with massive infiltration of eosinophil actually represents an important inflammatory reaction mediated by T cells and partially mast cells. In this model, some Kampo formulations and synthetic anti-allergic agents inhibited the IgE-mediated triphasic cutaneous reaction. The inhibitory effects of the Kampo formulations on the triphasic cutaneous reaction were divided into several groups according to the efficacies for IPR/LPR/vLPR. For instance, the group consisting of formulations such as Tokaku-joki-to (Tao-He-Cheng-Qi-Tang), Ji-zuso-ippo (Zhi-Tou-Chuang-Yi-Fan), Sho-sei-ryu-to (Xiao-Qing-Long-Tang) and Sho-saiko-to (Xiao-Chai-Hu-Tang) significantly inhibited IPR, LPR and vLPR (<I>i.e.</I> +/+/+ group that showed inhibitory effects against the triphasic response), similar to the effect of prednisolone as a positive control. Oral administration of Yokukan-san (Yi-Gan-San), an anti-psychosis drug in Kampo medicine, attenuated the isolation stress-exacerbated triphasic skin reactions in a dose-dependent manner, while it had almost no effect on the cutaneous reactions in the unstressed group-housed mice. On the other hand, the i.p. administration of diazepam, a classic benzodiazepine receptor agonist, suppressed the enhanced IPR and LPR in socially isolated mice, but surprisingly stimulated vLPR in both stressed and unstressed mice, differing from the efficacy of Yokukan-san.<BR>This article focuses on the anti-allergic properties of Kampo formulations and describes the effect of some Kampo formulations on IgE-mediated triphasic skin reaction in group-housed or socially isolated mice. We also discuss the mechanism of the inhibitory action and the importance of the formulation and the constituent drugs in determining the efficacy.
- 和漢医薬学会の論文
和漢医薬学会 | 論文
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