The antioxidative activity of Kangen-karyu extract delays senescence of human lung fibroblasts
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概要
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Replicative senescence (RS) of human diploid fibroblasts (HDFs) has become a classical model of aging and HDFs, such as WI-38 cells, display increased cellular oxidant production associated with RS. Several phenomena associated with RS are also observed in stress-induced replicative senescence (SIPS). In particular, SIPS of WI-38 cells caused by hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) is a useful and reasonable cellular aging model for evaluating the effects of potential anti-aging agents against oxidative stress. We used this well-established model to evaluate the anti-aging effect of Kangen-karyu, focusing on its antioxidant activity. Treatment of WI-38 cells undergoing SIPS caused by H<SUB>2</SUB>O<SUB>2</SUB> with Kangen-karyu extract significantly reduced reactive oxygen species (ROS) generation and lipid peroxidation levels. In addition, the intracellular GSH levels, reflecting cellular ROS generation, were reduced by treatment with Kangen-karyu extract. These results suggest that Kangen-karyu attenuated the age-associated increase of cellular oxidative damage. Moreover, Kangen-karyu extract normalized the G<SUB>0</SUB>/G<SUB>1</SUB> phase arrest and reversed the diminished cell viability resulting from exposure to H<SUB>2</SUB>O<SUB>2</SUB>. Furthermore, the extract prolonged the lifespan of WI-38 cells undergoing SIPS. This study suggests that Kangen-karyu may delay the aging process in cells undergoing SIPS by attenuating oxidative damage.
- 和漢医薬学会の論文
和漢医薬学会 | 論文
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