Expression analysis of obg, the gene controlling morphological development of Streptomyces griseus IFO 13189.
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概要
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The expression of the <I>obg</I> gene of <I>S.griseus</I>, which codes for a GTP-binding protein and controls morphological development of this organism, was studied by Western and Northern blot analyses. Western analysis revealed that Obg protein level sharply decreases just after the onset of aerial mycelium development or at the end of vegetative growth on agar plate cultures, indicating that expression of <I>obg</I> is regulated in a growth phase-dependent manner. The <I>obg</I> gene was found to be transcribed as a mRNA of 1.7 kb and its transcription was cut off just prior to the decrease of Obg protein, indicating that <I>obg</I> gene expression is controlled at the transcription level.
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