指紋検出に用いるニンヒドリン，インダジオン，DFO，ポリシアノ DMABおよび T.TEC が STR 型検査に及ぼす影響

概要

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Ninhydrin and its analogues are wildly used to detect latent fingerprints on porous materials. When the contrast of ninhydrin-developed fingerprints is not sufficient, indium chloride (InCl3) treatment has been used to improve their images. For non-porous materials, polycyanoacrylate p-dimethylaminobenzaldehyde (DMAB) method and tris thienyl europium chelate (T.TEC) method have recently been developed in Japan. In this study, we examined the effects of these latent fingerprint detection methods on short tandem repeat (STR) typing. Saliva and blood samples were attached to and dried on copy paper for the experiments of the ninhydrin, 1,2-indanedione, and 1,8-diazafluoren-9-one (DFO). Saliva and blood samples were also smeared and dried on glass slides for the experiments of the polycyanoacrylate DMAB, and the T.TEC. DNA was extracted from the samples, quantified by a real-time PCR assay, and the STR typing was performed using the Identifiler kit. Various ninhydrin solutions including the acetone and acetic acid solution tended not to reduce the DNA concentrations, and provided full STR profiles. Heating by putting an iron directly on the samples remarkably reduced the DNA concentrations for 30 seconds. It was recommended that direct-heating be prohibited and that the steam from an iron be used in the ninhydrin method. The InCl3, the indanedione, the DFO, the polycyanoacrylate DMAB and the T.TEC methods did not reduce or slightly reduced the DNA concentrations, but full STR profiles were obtained. The transfer membranes used in the InCl3 method gave low DNA concentrations because the samples were likely to remain in the residual portions on glass slides. The ultraviolet light (350 nm) used in the polycyanoacrylate DMAB and the T.TEC methods did not adversely affect the DNA concentration and the STR typing.
日本法科学技術学会の論文

指紋検出に用いるニンヒドリン，インダジオン，DFO，ポリシアノ DMABおよび T.TEC が STR 型検査に及ぼす影響

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概要

日本法科学技術学会 | 論文

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