Use of the Genome Profiling Method for the Identification of Saliva and Sweat Samples

概要

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The identification of body fluids at crime scenes provides critical evidence that can be used to prove the occurrence of a crime. In this study, the genome profiling (GP) method was utilized to identify saliva and sweat. We randomly amplified cDNA obtained through the RT-PCR approach using RNA samples extracted from saliva and sweat with four different random primers, and performed temperature gradient gel electrophoresis between 15-65°C. The Sp-2 primer was the only primer which generated the species identification dots (spiddos) in all body fluids. The numbers of spiddos found were 11.6±0.89 in saliva and 3.0±1.73 in sweat, and the body fluid type specific spiddos were obtained from electrophoresed gel. Along with previously reported data of semen and vaginal fluid, it was indicated that the GP method might distinguish those four kinds of body fluids. This novel assay is a simple and economical method. Therefore it may be an effective tool for the identification of body fluids at crime scenes. However, further detailed studies, including sensitivity, reproducibility, environmental effect and the effectiveness of detection from mixed stains are necessary before actual forensic investigations.
日本法科学技術学会の論文