Ｐｙ‐ＧＣによる植物細胞壁構成成分を指標とした植物分類法の検討
スポンサーリンク
概要
- 論文の詳細を見る
Identification of plant species is one of the clues, which is an important tool in criminal investigation. However, morphological test and DNA extraction are very difficult in minute plant pieces. Thus, it is necessary to develop a technique for classification and identification based on biological and chemical components, which does not require DNA analysis. This study analyzed the lignin of the cell wall components, which are products of secondary metabolism of a plant and also examined the species peculiarity and utility in forensic science. An analysis method of lignin is Pyrolysis-Gas chromatography (Py-GC). Analysis of trace amounts of dried plant samples using Py-GC shows results in waveform data, called pyrogram. Powdered samples each of 1.4 mg were dried, in all 22 plant species including Gramineae and Leguminosae were used for Py-GC. Thirteen characteristic peaks were chosen among a pyrogram of Gramineaes and it was converted into a bar graph and compared with other plant species. As a result, same plant species showed the similar pattern, and the same plant that was taken from a different place also showed the same pattern, and reproduction characteristics were recognized. Therefore, the possibility that difference in plant species is specific was shown by the pyrogram pattern. Even if it was the sample, in which DNA cannot be extracted, as for this method, a classification of a plant species was possible. Preprocessing of a sample is simple, as it has to only be dried. In conclusion, the application of this method in criminal investigation will involve swiftness and also forms a simple classification method for plant species.
- 日本法科学技術学会の論文
日本法科学技術学会 | 論文
- メチレンジオキシアンフェタミン(MDA)抗体の作製とその抗体の関連化合物との反応性
- メタンフェタミン摂取者尿中のρ-ヒドロキシメタンフェタミン抱合体濃度およびその加水分解条件の検討
- 耳介各部の形態分類と各部における形態特徴間の関連性に関する研究
- E-ウロカニン酸,L-チロシンを指標とした汗斑の証明
- 撮影角度の異なる画像間における耳介の個人識別