染毛の形態検査における横断面観察の応用

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With the spread of hair coloring, the number of cases where dyed hairs are examined as evidential samples is on the increase. In forensic casework, hair color observed with the naked eye and light microscope is an important characteristic for morphological examination, while the color of dyed hair is considered of little importance and sometimes is excluded from examination because it no longer has its original color. It is experientially recognized that peculiar morphology is often observed in the cross section of dyed hairs. However, making cross sections of hair is so laborious that such observations are rarely employed in actual casework, and nothing more than noting whether hair is dyed or not is generally done. Then, for effective application of hair cross sectioning to morphological examination of dyed hairs, the sectioning method for hair samples was improved and cross section morphologies of dyed hairs made with a newly improved method were compared among Japanese females. It requires a lot of time, labor and skill to make cross sections of hairs with the conventional hair cross sectioning method of using a celluloid plate as an embedding material. Cross sections of hairs can be made easily using the Technovit 7100, an embedding resin system based on glycol methacrylate. Cross sections made with the 'Technovit method' are of equal thickness and perpendicular to the hair shaft so that cross section morphologies such as a thickness and damage of cuticle, density of melanin granules and state of dyeing can be accurately appraised. In cross sections of dyed hairs collected from twenty Japanese females, several peculiar morphologies that give useful information for morphological comparison were observed.
日本法科学技術学会の論文

日本法科学技術学会 | 論文

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