Synthesis and Structure Analysis of Glucosylpsicose Produced by Cyclomaltodextrin Glucanotransferase
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概要
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Glucosylpsicose was synthesized by transglycosylation with α-cyclodextrin and D-psicose using cyclomaltodextrin glucanotransferase (EC 2.4.1.19; CGTase). The structure of glucosylpsicose was analyzed by HPLC to determine sugar composition and molecular mass, by methylation analysis using GC-MS and by α- and β-glucosidase treatments to determine linkage, and 1H- and 13C-NMR spectrometries to obtain the anomeric configuration of the glycosidic linkage. By chemical analysis, it was found that the structure of glucosylpsicose is 1-o-α-D-glucopyranosyl-D-psicose.
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