Cloning, Sequencing and Expression Analysis of an Endo-1,4-.BETA.-D-Xylanase Gene from Aspergillus sojae

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A xylanase gene was cloned from the cDNA library of Aspergillus sojae. The nucleotide sequence suggested that the mature enzyme is preceded by a 22 amino acid signal sequence and the molecular mass was predicted to be 32,403 Da. The deduced amino acid sequence of the enzyme exhibits good identity (98%) with that of Aspergillus oryzae xynF3 (T. Kimura et al.: Biosci. Biotechnol. Biochem., 66, 285-292, 2002), which is a enzyme that belongs to family 10 of the glycosyl hydrolases. The mature xylanase gene was expressed in Escherichia coli as a cellulose-binding domain tag fusion protein. The recombinant protein expressed high enzyme activity and the temperature stability was increased remarkably more with CBIND™ 100 Resin in the reaction mixture than was the case without the resin.
日本応用糖質科学会の論文