Hydrothermal synthesis of hydroxyapatite nanoparticles and their protein adsorption behavior
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概要
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Protein adsorption on hydroxyapatite particles with different morphologies was investigated. The nano-sized hydroxyapatite (nano-HAp) was prepared using calcium nitrate tetrahydrate [Ca(NO3)4·4H2O] and diammonium hydrogen phosphate [(NH4)2HPO4] by using a hydrothermal method with varying synthesis temperature and pH conditions. The adsorption properties of proteins onto nano-HAp were studied using three types of proteins: bovine serum albumin (BSA, an acidic protein), myoglobin (MGB, a neutral protein), and lysozyme (LSZ, a basic protein). The adsorption amount of BSA and LSZ per unit specific surface area of nano-HAp increased as the crystallinity improved. In contrast, the improvement in the crystallinity of HAp decreased adsorption affinity to MGB. This adsorption behavior on nano-HAp would be considered by the specific binding of the C- or P-site on HAp towards acidic or basic proteins, respectively.
- 公益社団法人 日本セラミックス協会の論文
公益社団法人 日本セラミックス協会 | 論文
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