Comparison of effects of PKA catalytic subunit on Ih and calcium channel currents in rat dorsal root ganglion cells
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概要
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We investigated whether PKA-induced phosphorylation was involved in regulation of hyperpolarization-activated current (<I>I<SUB>h</SUB></I>) in rat dorsal root ganglion (DRG) cells. We examined the effect of the catalytic subunit of PKA (PKAc) on <I>I<SUB>h</SUB></I> and confirmed an effect of PKAc on Ca<SUP>2+</SUP> channel currents carried by Ba<SUP>2+</SUP> (<I>I<SUB>Ba</SUB></I>) in identical neurons as a positive control of PKA activity. After the start of recording, amplitudes of <I>I<SUB>Ba</SUB></I> gradually decreased (rundown). An intracellular application of ATP reduced the rundown of <I>I<SUB>Ba</SUB></I> and induced a depolarizing shift of <I>I<SUB>h</SUB></I> activation. The former was partially reversed by PKI but the latter was not affected. An intracellular application of PKAc also prevented the rundown of <I>I<SUB>Ba</SUB></I> and this effect was potentiated by okadaic acid (OA). The application of PKAc and OA in combination did not change the electrophysiological properties of <I>I<SUB>h</SUB></I> although a potentiating effect on <I>I<SUB>Ba</SUB></I> was observed in the same neurons. The application of 2-mM ATP in addition to PKAc and OA did not result in an additional potentiation of <I>I<SUB>Ba</SUB></I>, but shifted the activation curve of <I>I<SUB>h</SUB></I> positively. These results suggested that PKA-induced phosphorylation was not involved in the modulatory mechanisms of <I>I<SUB>h</SUB></I> in rat DRG neurons.
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