Simultaneous detection of Bacteroides fragilis group species by leuB-directed PCR
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概要
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<I>Bacteroides</I> species, saccharolytic Gram-negative obligate anaerobes, are frequently isolated from human infections such as peritonitis, abscesses and bacteremia. Among the species in the genus <I>Bacteroides</I>, the species called "<I>B. fragilis</I> group" are particularly involved in human infections and are medically important because they account for a major part of anaerobic isolates from clinical specimens. The purpose of this study was to develop PCR primers that specifically and simultaneously amplify the β-isopropylmalate dehydrogenase gene <I>leuB</I> in <I>B. fragilis</I> group species. We determined partial nucleotide sequences of <I>leuB</I> genes and compared them in seventeen strains of nine <I>B. fragilis</I> group species, and the regions that are conserved among <I>Bacteroides</I> strains but different from other species were used as a <I>B. fragilis</I> group-specific PCR primer set, BacLBF-BacLBR. Specificity tests of the primer set using 52 phenotypically characterized strains and 75 isolates from rat feces showed only one case each of false-positive and false-negative. The detection limit of the <I>leuB</I>-directed PCR using BacLBF and BacLBR was 3.9×10<SUP>3</SUP> colony-forming units. These results indicate that <I>leuB</I> amplification using BacLBF and BacLBR is a useful tool for rapid diagnosis of <I>Bacteriodes</I> infection and for rapid differential diagnosis of anaerobic infections. J. Med. Invest. 52: 101-108, February, 2005
- 国立大学法人 徳島大学医学部の論文
国立大学法人 徳島大学医学部 | 論文
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