N-2-Mercaptopropionyl Glycine Blocks Ischemic Tolerance and Synthesis of Heat Shock Protein 70 in Rabbit Hearts
スポンサーリンク
概要
- 論文の詳細を見る
Since oxidative stress is reported to be one of the mediators of synthesis of heat shock protein 70 (HSP70) induced by hyperthermia, we investigated the effects of N-2-mercaptopropionyl glycine (MPG), a diffusible antioxidant, in in vivo rabbit model of heat stress (HS) preconditioning. Three groups of rabbits were studied: Group I, control rabbits treated with anesthetic alone; Group II, rabbits subjected to HS by raising core temperature to 42°C for 15 min; Group III, given MPG (50mg/kg/hr i.v.), beginning 30 min prior to HS and continued up to 15 min post HS. Twenty-four hours later, all animals were treated with 30 min of the left anterior descending coronary artery occlusion followed by 180 min of reperfusion under ketamine/xylazine anesthesia. Risk area was delineated by Evans blue and infarct size determined by tetrazolium staining. The risk area ranged from 59.3±5.8 % to 66.3±3.4 % with no significant difference among all the groups. Infarct size/area risk was 53.6±5.9 % in control rabbits, and decreased significantly to 26.7±4.3 % in the HS hearts. Treatment with MPG of HS rabbits resulted in a significant increase in the infarct size (48.6±11.8 %). The results show that prior HS significantly reduced infarct size (P<0.05) which was inhibited by MPG (P<0.01). Western blot analysis revealed significant synthesis of HSP70 in HS rabbit hearts and that synthesis was blocked with MPG. We conclude that oxidative stress is one of critical mediators of HS induced myocardial protection in vivo and trigger expression of HSP70.
- 日本循環制御医学会の論文
日本循環制御医学会 | 論文
- 心刺創に対する経食道心エコー図の有用性
- Ｍａｙｅｒ波帯域における脈波伝播時間－心拍数間の相互相関を用いた情動反応の定量化
- 癌性サブイレウスを呈した急性冠症候群の2手術症例
- 釘打ち器による左室，右室損傷の1例
- ST depression during thoracic cavity irrigation in a patient undergoing fistula closure for pneumothorax