Preliminary Studies of in vivo and ex vivo Implantations Accompanied by Transgenes
スポンサーリンク
概要
- 論文の詳細を見る
Tissue engineering is the three-dimensional assembly over time of vital tissues and organs by a process involving cells, signals and extracellular matrix. Use of endogenous stem/progenitor cells combined into designed scaffolds with cytokines or growth factors for local delivery has been demonstrated as a discipline of more effective tissue engineering. However, several barriers to effective and safe local delivery of proteins in vivo have been identified because of their short life, high costing, difficulty to maintain full bioactivity and high-dose local delivery associated with both local and systemic toxicity. To overcome these barriers, gene therapy has emerged as an effective approach by which therapeutic proteins could be carried as genes. The preliminary studies introduced here are to explore the feasibility of our designed tissue engineering constructs using our modified gene activated matrix (GAM) with calcium phosphate and transgene for BMP-2 for in vivo, and periosteal cell culture with a collagen sponge and transgene for BMP-2/VEGF for ex vivo.Our modified GAM tremendously bridged the bone defect with 12µg plasmid encoding for BMP-2 after 6 weeks, which was followed by mechanically strong bone replacement. And the second study implicated that the implanted collagen sponges harboring periosteal cells with BMP-2/VEGF contained the most alkaline phosphatase activity, enhancement of many of osteogenic gene expressions and prominent angiogenesis.
- 日本再生歯科医学会の論文
日本再生歯科医学会 | 論文
- P14.ジルコニアおよびチタン上における骨芽細胞様細胞の増殖(一般演題抄録,第5回日本再生歯科学会)
- P48.歯牙再植後における歯根膜細胞の増殖と動態(一般演題抄録,第5回日本再生歯科学会)
- P45.出生前後でマウス歯乳頭において発現減少をきたす遺伝子の検索(一般演題抄録,第5回日本再生歯科学会)
- P24.歯肉切除後のマウス再生付着上皮におけるlaminin-γ_2,integrin-β_4の免疫局在(一般演題抄録,第5回日本再生歯科学会)
- P23.ラット口腔粘膜初代培養細胞の接着・遊走におけるlaminin-γ_2、integrin-β_4、integrin-α_3の発現(一般演題抄録,第5回日本再生歯科学会)