In vitro Evaluation of Short-Term Storage Solution for Bone Grafts in Dental Implant Surgery
スポンサーリンク
概要
- 論文の詳細を見る
Bone grafts are frequently applied to patients with severe bone defects as part of implant surgery. Normally, bone particles collected during the drilling process and other fragments obtained from the donor site are temporarily stored in saline. However, it is doubtful whether saline is suitable for implant surgery. To address this issue, we examined the effects of three different solutions on cells of osteoblastic lineage. Rat bone marrow cells were preserved in normal saline, α minimum essential medium, or Hanks' balanced salt solution (HBSS) for 1 hour, after which the cells were inoculated to culture plates. At Days 7, 10 and 14, alkaline phosphatase activity and DNA amount were measured and cell viability was assessed. These results indicate that HBSS has the potential to provide superior storage conditions than saline for osteoblast differentiation. Currently, saline is conventionally used in dental implant surgery; however, HBSS is more suited to maintaining osteogenic cells for bone grafting.
- 日本再生歯科医学会の論文
日本再生歯科医学会 | 論文
- P14.ジルコニアおよびチタン上における骨芽細胞様細胞の増殖(一般演題抄録,第5回日本再生歯科学会)
- P48.歯牙再植後における歯根膜細胞の増殖と動態(一般演題抄録,第5回日本再生歯科学会)
- P45.出生前後でマウス歯乳頭において発現減少をきたす遺伝子の検索(一般演題抄録,第5回日本再生歯科学会)
- P24.歯肉切除後のマウス再生付着上皮におけるlaminin-γ_2,integrin-β_4の免疫局在(一般演題抄録,第5回日本再生歯科学会)
- P23.ラット口腔粘膜初代培養細胞の接着・遊走におけるlaminin-γ_2、integrin-β_4、integrin-α_3の発現(一般演題抄録,第5回日本再生歯科学会)