Effects of photodynamic therapy using talaporfin sodium (Laserphyrin®) on wound healing in an animal model

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Background: Photodynamic therapy (PDT) is an effective laser treatment for locally advanced carcinoma and is promisingas neoadjuvant chemotherapy before surgery. The aim of this study was to clarify the adverse effects of PDT using a photosensitizer,talaporfin sodium (Laserphyrin®), for wound healing.　Methodology: For PDT, a laser light with a wavelength of 660 nm and a frequency of 10 Hz with a total energy fluency of60 J/cm2 was used. Macroscopic and histological findings of wound healing after PDT were examined in vivo (4-week-old maleBALB/c mice).　Results: In Model 1, in which skin was cut at 0, 3, 7 days after PDT (n=3, each), wounds were similarly healed 7 days aftercutting in all groups, and regenerating epithelium and the number of fibroblasts on histological findings were not different. InModel 2, in which skin defects were created before or after PDT, the size of the defects was larger at day 7 in the groups withskin defects before or after PDT in comparison with groups with no PDT.　However, macroscopic wound healing at day 14 was complete in all groups and there were no significant differences amongthe groups by this point. Histological findings of skin defects at day 14 showed no significant difference in terms of regeneratingepithelium and number of fibroblasts in each group with or without PDT.　Conclusions: PDT did not influence wound healing and can be safely applied before surgical therapy.
長崎大学医学部の論文