Differential Chromosome Banding and RAPD Analysis of Four Varieties of Capsicum frutescens L.

概要

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Karyotype and RAPD analysis of four varieties of Capsicum frutescens L. (Chilli), namely BARI Chilli-1, Bogra Chilli, Binti Chilli, and Coarse Chilli, were conducted. These four varieties were found to possess 2n=24 chromosomes. The total length of 2n chromosome complement was highest (86.35 μm) in the Coarse Chilli and lowest (38.57 μm) in the Bogra Chilli. The centromeric formulae of these four varieties were different; they were 20m+2sm+2ac for BARI Chilli-1, 21m+3sm for Bogra Chilli, 19m+2sm+3ac for Binti Chilli, and 10m+14sm for Coarse Chilli. The range of individual chromosome length was largest (1.92–4.84 μm) in the Coarse Chilli and smallest (0.84–2.22 μm) in the Bogra Chilli. Heteromorphicity in respect of centromeric position in a member of pair XI of Bogra Chilli and Binti Chilli suggested the occurrence of deletion. Four CMA-bands were found in BARI Chilli-1 and Bogra Chilli, whereas two chromosomes of the Coarse Chilli entirely fluoresced with CMA. No CMA band was found in the Binti Chilli. Each variety had a definite RAPD fingerprinting pattern. The Coarse Chilli had several unique DNA fragments. Therefore, the four variety of Capsicum frutescens L. could be characterized with the help of karyotype and RAPD analysis.
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