Different Hematopoietic Reconstruction Abilities of Transplanted Cells from Bone Marrow, Spleen, Liver and Peripheral Blood

概要

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Cells from different lineages may exert a different growth capacity following transplantation. To compare the efficacy of different cells on rebuilding hematopoietic function, peripheral leukocytes, spleen cells, liver cells and bone marrow cells derived from GFP transgenic male mice were transfused into irradiated female mice. GFP-positive cells in the peripheral blood of female recipients were detected 20, 40, or 60 days after transplantation. At the 180th day after transplantation, mice were sacrificed and bone marrow cells were collected to detect the Y chromosome-positive rate. Results showed that GFP-positive cells appeared as early as 20 days after transplantation in peripheral blood, and some cells had differentiated to CD4+ or CD8+ T cells. The differentiation rate was different among the four types of donor cells. The Y chromosome-positive rate in bone marrow and the GFP-positive rate in peripheral blood were positively correlated. Although all 4 types of cells were able to rebuild hematopoietic function, spleen and bone marrow cells showed the highest capacity, followed by liver cells. Leukocytes showed the least reconstitution efficacy.
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