Assessment of the Genotoxicity of 1,2-Dichloropropane and Dichloromethane after Individual and Co-exposure by Inhalation in Mice
スポンサーリンク
概要
- 論文の詳細を見る
Objective: Occurrence of cholangiocarcinoma was recently reported at a high incidence rate among the employees working for an offset printing company in Osaka, Japan. 1,2-Dichloropropane (1,2-DCP) and dichloromethane (DCM) are suspected to be the causes of the cancer, as they had been used as ink cleaners in large amounts. However, it is not clear whether these chlorinated organic solvents played a role in the occurrence of cholangiocarcinoma or why the incidence rate is so high among the workers in this industry. To provide possible evidence for this severe occupational problem, we investigated the genotoxic effects of 1,2-DCP and DCM. Methods: Male B6C3F1 and gpt Delta C57BL/6J mice were exposed by inhalation to the individual solvents or both solvents at multiple concentrations including the levels that were possibly present in the workplaces. The genotoxicity was analyzed by Pig-a gene mutation and micronuclei assays in peripheral blood and gpt mutation and comet assays in the livers of mice after repeated inhalation of 1,2-DCP or/and DCM. Results: The Pig-a mutant frequencies and micronuclei incidences were not significantly increased by exposure of either 1,2-DCP or/and DCM at any concentration, suggesting there was no genotoxic potential in bone marrow for both solvents. In the liver, DNA damage, as measured by the comet assay, was dose dependently increased by 1,2-DCP but not by DCM. The gpt mutant frequency was 2.6-fold that of the controls in the co-exposure group. Conclusions: These results indicate that 1,2-DCP showed stronger genotoxicity in the liver and that the genotoxic effects were greatly enhanced by simultaneous exposure to DCM.
著者
-
SUZUKI Tetsuya
Division of Marine Life Sciences, Grauate School of Fisheries Science, Hokkaido University
-
Suda Megumi
Division Of Health Effects Research National Institute Of Industrial Health
-
Wang Rui-sheng
Division Of Health Effects Research National Institute Of Industrial Health
-
Yanagiba Yukie
Division Of Health Effects Research Japan National Institute Of Occupational Safety And Health
-
Suda Megumi
Division of Health Effects Research, National Institute of Occupational Safety and Health
関連論文
- Detoxifying Effect of Desalinated Deep Seawater on Organotin-poisoned Euglena gracilis(Food & Nutrition Science)
- Effect of Cathodic Solution Produced by the Electrolysis of a Dilute NaCl Solution on the Oxidation of Linoleic Acid, Linoleoyl Acylglycerols, Soybean Phosphatidylcholine and α-Tocopherol
- Inhibitory Effect of Cathodic Solution Produced by the Electrolysis of a Dilute NaCl Solution on the Oxidation of Squalene, Vitamin A and β-Carotene
- Effects of Toluene on Regulation of Adenylyl Cyclase by Stimulation of G-Protein-Coupled Receptors Expressed in CHO Cells
- P-53 Changes in brain muscarinic receptors induced by toluene(Proceedings of the 27th Annual Meeting)
- 22B-10-2 Effects of Toluene Exposure on Brain Cholinergic Neurons.
- Diurnal variation of cadmium-induced mortality in mice
- Influence of injection timing on severity of cadmium-induced testicular toxicity in mice
- Assessment of the Genotoxicity of 1,2-Dichloropropane and Dichloromethane after Individual and Co-exposure by Inhalation in Mice