Effects of butane-2,3-dione thiosemicarbazone oxime on testicular damage induced by cadmium in mice
スポンサーリンク
概要
- 論文の詳細を見る
Our group of studies investigated the action of butane-2,3-dione thiosemicarbazone oxime against the testicular damage caused by cadmium chloride (CdCl2) in mice. Mice received a single injection of CdCl2 (5 mg/kg, intraperitoneally) and, after thirty minutes, the oxime (10 mg/kg, subcutaneously) was administered. Twenty four hours after the last administration, the animals were killed by cervical dislocation and the testes and serum were removed for analysis. The parameters determined were δ-aminolevulinate dehydratase (δ-ALA-D), myeloperoxidase (MPO), glutathione-S-transferase (GST) and glutathione peroxidase (GPx) activities. The levels of thiobarbituric acid-reactive substances (TBARS), nonprotein thiols (NPSH), ascorbic acid, cadmium and testosterone were also determined. In addition, histological analysis and cytokines quantification (IL-1, IL-6, IL-10, TNF-α and IFN-γ) were performed. Our results demonstrated that the oxime was effective in restoring the inhibition in δ-ALA-D activity induced by CdCl2. The activation of MPO and increase in IL-1, IL-6, TNF-α and IFN-γ levels induced by CdCl2 were also reduced by oxime. IL-10, which was reduced by cadmium, was restored by oxime administration. In addition, the oxime was effective in restoring the increase in TBARS levels and the reduction on NPSH levels induced by CdCl2. Our results demonstrated that oxime was effective in containing the histological alterations induced by CdCl2. In addition, oxime was able to increase the testosterone levels, reduced by cadmium exposure. In conclusion, the oxime tested was effective in reducing the testicular damage induced by CdCl2 in mice. The beneficial effects of this oxime are related to its antioxidant and anti-inflammatory action.
- 一般社団法人 日本毒性学会の論文
一般社団法人 日本毒性学会 | 論文
- No carcinogenicity of ethyl tertiary-butyl ether by 2-year oral administration in rats
- Genotoxicity evaluation of chlorpyrifos: a gender related approach in regular toxicity testing
- Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ9-tetrahydrocannabinol in human breast cancer cells
- Increased production of reactive oxygen species by the vacuolar-type (H+)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells
- Evaluation of cytotoxic potential of cored soft contact lenses with adsorbed active ingredients from over-the-counter eye drops