HDL2 Can Inhibit Further Oxidative Modification of Partially Oxidized LDL
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Aim: To investigate whether HDL2 can inhibit further oxidative modification of partially oxidized LDL (ox-LDL) by interrupting the chain oxidation reaction after lipid hydroperoxides (LOOH) formation. <BR>Methods: Following incubation of LDL 400 μg protein/mL phosphate-buffered saline with Cu2+ for 1.75 h (defined as 0 min), incubation was continued after adding HDL2 200 μg protein/mL or HDL2 800 μg protein/mL to give both ox-LDL+HDL2 200 μg protein/mL or ox-LDL+HDL2 800 μg protein/mL. As a control, ox-LDL 200 μg protein/mL and native LDL were prepared. Each sample was subjected to agarose gel electrophoresis and the LOOH in each sample was measured.<BR>Results: When the electrophoretic mobility of native LDL was designated 1, the relative electrophoretic mobility (REM) of ox-LDL increased significantly over time.The REMs of ox-LDL+HDL2 800 μg protein/mL from 10 min to 9 h were significantly lower than the REM of ox-LDL at the respective times (p<0.01). LOOH of ox-LDL+HDL2 800 μg protein/mL at 1, 3, 6 and 9 h was significantly higher than LOOH in ox-LDL at the respective times (p<0.01). The results of ox-LDL+HDL2 200 μg protein/mL were almost the same but to a lesser extent than the results of ox-LDL+HDL2 800 μg protein/mL.<BR>Conclusion:The present findings suggest that HDL2 can inhibit further oxidative modification of partially oxidized LDL by interrupting the chain oxidation reaction after LOOH formation in a concentration-dependent manner.
- 一般社団法人 日本動脈硬化学会の論文
一般社団法人 日本動脈硬化学会 | 論文
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