A Promoter in the Novel Exon of hPPARγ Directs the Circadian Expression of PPARγ
スポンサーリンク
概要
- 論文の詳細を見る
Aim: PPARγ (peroxisome proliferator-activated receptor γ) is a member of the nuclear receptor superfamily of ligand-activated transcription factors that regulate the expression of genes associated with lipid metabolism. Herein, we show that expression levels of the novel PPARγ transcript exhibit circadian oscillation. To study the mechanisms controlling PPARγ expression, a novel PPARγ gene promoter was cloned and characterized.<BR>Methods: We analyzed the novel PPARγ promoter by luciferase reporter assays and gel shift analysis.<BR>Results: Surprisingly, it was not an intron but rather the novel first exon of PPARγ that was found to have functional minimal promoter activity. Luciferase reporter assays and gel shift assays revealed that the novel first exon is essential for novel PPARγ promoter activation and that DBP (albumin gene D-site binding protein) and E4BP4 (E4 promoter A binding protein 4) bind directly to D-sites in the novel first exon.<BR>Conclusion: Our results demonstrate that the PAR-bZIP (bZIP, basic leucine zipper) family and E4BP4 are the main regulatory factors involved in oscillation of novel PPARγ expression. This regulatory mechanism clearly differs from that of the circadian expression of PPARα.
- 一般社団法人 日本動脈硬化学会の論文
一般社団法人 日本動脈硬化学会 | 論文
- Effects of Lysosomal Protease Inhibitors on the Degradation of Acetylated Low Density Lipoprotein in Cultured Rat Peritoneal Macrophages
- Genomic Structure and Mapping of Human Orphan Receptor LXR Alpha : Upregulation of LXRa mRNA During Monocyte to Macrophage Differentiation
- The Gene Expression Profile of Human Umbilical Vein Endothelial Cells Stimulated by Tumor Necrosis Factor a Using DNA Microarray Analysis
- Participation of T Lymphocytes and Macrophages in Atherogenesis
- Immunohistochemical and Quantitative Analysis of Cellular and Extracellular Components of Aortic Atherosclerosis in WHHL Rabbits