The Study of Circular Dichroic Difference Spectra Applied to Polynucleotides and Ribonucleic Acids
スポンサーリンク
概要
- 論文の詳細を見る
The CD difference spectrum was applied to the elucidation of the conformational change in RNA as well as the estimation of its base pair content. First, it was revealed that the RDV RNA recooled form was composed of the fully reconstructed G–C pairs and partially destroyed A–U pairs, being in good agreement with the result of RNase treatment. Second, the CD difference spectrum of unfractionated <I>t</I>RNA from an extreme thermophile was quite similar to that of <I>t</I>RNA<SUB>f</SUB><SUP>met</SUP> from <I>E. coli</I> and random copolymer poly (G, C), showing that the thermophile <I>t</I>RNA is rich in G–C pairs and is consistent with the result of <I>T</I><SUB>m</SUB> measurement. The limitedly hydrolyzed fragments of <I>t</I>RNA<SUB>f</SUB><SUP>met</SUP> by RNase T<SUB>1</SUB> and their equimolar complexes were also studied. The CD and CD difference spectra of 3′-three-quarter molecules (fragment L, M<SUB>3</SUB>, M<SUB>2</SUB>) were almost identical but those of their equimolar complexes with 5′-quarter molecule (fragment N) were different from each other. Especially N+M<SUB>2</SUB> revealed a quite different CD pattern from the rest. By combining the work of electrophoresis it was deduced that some false complexes were formed in N+M<SUB>2</SUB> due to incompleteness of base pairs in the diHU arm.
- 社団法人 高分子学会の論文
社団法人 高分子学会 | 論文
- Preparation and Characterization of Pseudopoly(trans-4-hydroxy-L-proline ester).
- Crystallite Sizes and Lattice Distortions of Gel-Spun Ultra-High Molecular Weight Polyethylene Fibers.
- Temperature Dependence of Viscosities and Potentiometric Titration Behavior of Aqueous Poly(acrylic acid) and Poly(methacrylic acid) Solutions.
- Study of drag reduction due to aqueous sodium polyacrylate by the rolling ball method.
- Drag-reducing effect in solutions of very high molecular weight polystyrene by the rolling ball method.