Enzymeimmunoassay for determination of cortisol in miniature pig.

概要

論文の詳細を見る
The measurement of cortisol in miniature pig blood plasma was examined with an enzymeimmunoassay where peroxidase was used as the enzyme labeling hormone. The antiserum used was Cortisol-3-CMO-HRP for the enzyme labeling hormone with respect to Cortisol-3-CMO-BSA. The proper dilution magnification of antiserum is 201, 000-242, 000 times, and 200, 000 times was used. Standardcurve was in the range of 0.01-50ng, and coefficient of variation (n=6) of the combination rate was 2.32-11.67%. Measurement sensitivity was 0.012ng/well. Recovery rates of cortisol in miniature pig blood plasma was 96.0±5.5% of the averages and the coefficient of variation was 10.4%. Inter-assay coefficient of variation of 3 kinds of male miniature pig plasma (n=6) in which the concentration of cortisol was different from was 5.4-8.8%, which were 7.2% of the averages, and the intra-assay coefficient of variation was 6.7-11.4%, which were 9.0% of the averages. Cortisol concentration in miniature pig blood plasma until the fifth day was measured from the end of pregnancy to post partum by using this method. It tended to it increase gradually the day as the delivery date approached, and the highest value was shown on the day of delivery at 90.4ng/ml, and three days later decreased to about 25ng/ml. From thease resorts, it was possible that Cortisol concentration in miniature pig blood plasma by using this method.
日本養豚学会の論文