In Vivo HVJ-Liposome Mediated gene Transfer into Adult Rat Liver.
スポンサーリンク
概要
- 論文の詳細を見る
We examined a simple and highly efficient <I>in vivo</I> method of gene transfer into rat hepatocytes using Hemagglutinating Virus of Japan (HVJ) and nuclear protein. The E. coli β-galactosidase (β-gal) gene was encapsulated in liposomes and incubated with HVJ to form vesicle complexes. These vesicle complexes were introduced into the rat liver portal injection. These injection methods were also performed on rats which had undergone partial hepatectomy 24 hrs prior to the injection. In the rats which received portal injection, 31.9±11.5% of the hepatocytes were stained with X-gal. This expression was bardly detectable 28 days after injection. However, in the partially hepatectomyzed rats, more than 20% of hepatocytes expressed β-gal protein 28 days after injection. The transcription of β-gal m-RNA was comfirmed by RT-PCR. We conclude that this gene transfer method is efficient andwould facilitate the development of genetic treatments for various liver diseases.
- 一般社団法人 日本消化器外科学会の論文
一般社団法人 日本消化器外科学会 | 論文
- A Case of Traumatic Injury of the Retrohepatic Inferior Vena Cava with Severe Liver Rupture.
- Cytoprotective Action of Co-enzyme Q10 and Ulinastatin on the Ischemic Damage of the Liver Following in Vivo Cold Infusion
- A Case Report of Lesser Omental Cyst.
- The Significance of Resection Range and Surgical Margin for Hepatocellular Carcinomas.
- Post transfusion hepatitis in heapatectomized patients.