Quantitation of Urinary Glutathione S-Transferase in Diabetic Nephropathy.
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概要
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The present study was conducted to examine whether urinary glutathione S-transferase (GST) activity is correlated with the degree of progression of diabetic nephropathy (study1) and whether the renal tissue distribution of GST reflects the renal damage in rats with streptozotocin (STZ)-induced diabetes (STZ rats)(study 2). Concentrating urine samples by freeze-drying enabled us to quantitate urinary GST activity. In study 1, urinary GST activity was significantly higher in the diabetic patients than in the healthy controls and was significantly correlated with the urinary albumin excretion index, and the N-acetyl-β-D-glucosaminidase and β<SUB>2</SUB>-microglobulin levels. In study 2, immunochemical staining using anti-GST Ya antibody revealed that basic GST (GST Ya) was uniformly distributed in the proximal tubules, but it was not detected in either the glomerular region or the distal tubules. Basic GST was also detected in the proximal tubules of the STZ rats, however its intensity was clearly heterogeneous. Insulin treatment normalized the tissue distribution of basic GST in the kidneys of STZ rats. Acidic GST (GST Yp) stained positive in the outer medulla and there were no differences among the three groups.<BR>Thus, elevated urinary GST activity in diabetic patients may reflect increased excretion of GST due to increased permeability of the proximal tubular cell membrane.
- 一般社団法人 日本糖尿病学会の論文
一般社団法人 日本糖尿病学会 | 論文
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