Age-related Changes in Insulin Action and Binding of <SUP>125</SUP>I-insulin to Cultured Human Skin Fibroblasts
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Using human skin fibroblasts, adapted in cell culture, age-related changes in cellular responsiveness to insulin and binding of <SUP>125</SUP>I-insulin to the cells were studied.<BR>The mean age of the skin donors was 19 yr in the case of the young group of cells (N=4) and 75 yr in the aged group (N=5).<BR>Experiments using the cells in the growth phase, in which the cellular responsiveness to insulin was more marked than in the phase of confluent monolayer, yielded the following results.<BR>1) The insulin stimulated glucose uptake was significantly smaller in the cells from aged donors than in those from young donors.<BR>2) The conversion of glucose to CO<SUB>2</SUB> was also significantly smaller in the cells from aged donors than in those from young donors.<BR>3) The maximal specific binding of <SUP>125</SUP> I-insulin to the cells from aged donors was significantly smaller than that to the cells from young donors.<BR>4) No significant difference in the effect of insulin on the binding of <SUP>125</SUP> I-insulin was observed between the young and aged groups of cells.<BR>5) Also, no significant differences between the two groups of cells were observed in the effects of time, temperature and pH on the binding of <SUP>125</SUP>I-insulin to the cells.<BR>These results suggest that a decrease in the number of binding sites of insulin occurs with advancing age without qualitative changes, and that this age-related change in binding sites of insulin represents one of the possible mechanisms for the age-related reduction in cellular responsiveness to insulin.
- 一般社団法人 日本糖尿病学会の論文
一般社団法人 日本糖尿病学会 | 論文
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