Studies on the Insulin-tike Activity (ILA) in Serum:Part I. Relation of Insulin Degrading Enzyme to Discrepancy of Insulin Activities between IRI and ILA
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The relation of insulin-degrading enzyme to the cause of discrepancy of insulin activities between immunological and biological determinations was studied.<BR>For the purpose of determination of insulin degradation, a separation with a thin-layer chromatograph and qualitative and quantitative determinations with a chromatoscannogram of degradation products derived from <SUP>131</SUP>I-labeled insulin by muscle tissue were performed.<BR>Under the conditions of the measurement of insulin-like activity (ILA) using a hemidiaphragm, the accumulative residual percentage of <SUP>131</SUP>I-labeled insulin (250μU) was calculated as 42.2 % of the initial insulin concentration in Krebs-Ringer bicarbonate buffer solution at 37°C for 120 minutes, while the accumulative residual percentage of the same dose of 1311-labeled insulin added to normal human serumbuffer solution was calculated as 85.6 %. The fact indicates that ILA in serum is expressed as an approximately two fold concentration by calculations using the insulin standard curve. This could be one of the causes of the discrepancy of insulin activities between IRI and ILA in seum.<BR>The addition of the degradation product of insulin induced by means of muscle tissue to the mixture of insulin and hemidiaphragm in vitro inhibited the insulin degrading enzyme activity and also reduced to some extent the biological effect of insulin on the diaphragm. This suggests that the degradation product is competitive with insulin probably against the membrane of muscle cells.
- 一般社団法人 日本糖尿病学会の論文
一般社団法人 日本糖尿病学会 | 論文
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