Alterations in Secretion of Salivary Proteins due to Surgical Stress in Mice.

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Alterations in secretion of salivary proteins due to surgical stress were experimentally studied. Saliva stimulated by pilocarpine was collected from the oral cavity of adult ICR male mice after surgical procedures. The simple surgical procedures, which included skin incision, development of a flap, and suturing, were performed under pentobarbital anesthesia. Serum corticosterone levels were elevated in the animals following the operation, due to surgical intervention. Salivary protein concentrations increased after surgical procedures. Kallikrein activities in the saliva in surgically treated animals were all markedly elevated. In contrast, saliva amylase activities relative to the salivary proteins were reduced in surgically treated animals. Salivary protein separation patterns on SDS-PAGE from surgically treated animals differed significantly from control mice and mice given a β-adrenergic agonist (isoproterenol), but they were very similar to those from mice given an α1-adrenergic agonist (phenylephrine). Pretreatment with prazosin (an α1-adrenergic antagonist) reduced surgically induced alterations in the separation pattern of salivary proteins. The results suggest that surgical stress enhances the secretion of salivary proteins, due mainly to α1-adrenergic receptor stimulation, in mice.
特定非営利活動法人日本口腔科学会の論文