Function of S-100 Protein with Secretion of Granular Convoluted Tubule in Rat Submandibular Gland. Immunoelectron Microscopic Study.:Immunoelectoron Microscopic Study
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Granular convoluted tubules in rat submandibular glands consist of granular cells (GC), pillar cells (PC) and transition cells (TC), and the granular cells show secretory granules which contain epidermal growth factor (EGF) and other biologically active peptides. The secretory granules of GC are released by alpha adrenergic stimulation. At the immunohistochemical level, PC and TC show an intense immunoreactivity for S-100 protein and calmodulin, the calcium binding proteins.<BR>In the present study, immunohistochemical profiles of EGF and S-100 protein were evaluated in rat submandibular glands following administration of methoxamine (MX) to elucidate the secretory mecha-nisms in granular convoluted tubules and striated ducts (SD) using light and electron microscopy. In addition, S-100 protein in the submandibular gland and secreted saliva was evaluated by EIA.<BR>Following administration of MX, the secretory granules of GC were released into the lumen of the ducts and the number of granules in GC was markedly decreased. Smooth surfaced blebs and separating zones were observed in the apical cytoplasm of GC and SD. Immunoelectron microscopy showed immunogold particles for S-100 protein in the blebs and cytoplasm of GC and SD, as well as those for EGF in the foamy, small granules and secretory pools of GC. Immunohistochemically, EGF was not detected in the GCT after 30 minutes, and EIA of the secreted saliva demonstrated S-100 proteins after 60 minutes of MX administration. The results of the present study suggest a possible role of S-100 protein in calcium signaling in the secretory mechanism of GCT and striated ducts.
- 特定非営利活動法人 日本口腔科学会の論文
特定非営利活動法人 日本口腔科学会 | 論文
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