Establishment and Characterization of Four Human Oral Cancer Cell Lines.

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In the present study, we tried to establish cell lines from 16 patients with squamous cell carcinoma of the oral cavity and maxillary sinus by a modified primary culture method. Finally, four novel cell lines were established, of which HOC 512 was from primary lesion of the maxillary sinus carcinoma, and HOC 1119 and HOC 1-19, and HOC 519 were from neck lymph-node metastases of tongue cancers and of cancer of oral floor, respectively. These cell lines have been cultivated more than one year and are in good condition. Moreover, we tested whether or not these cells could grow in a proteinfree medium of PF 86-1. Consequently, two cell lines were able to propagate serially in a protein-free condition and they were named HOC 519 PF and HOC 1-19 PF. It was also investigated whether or not these cells carry an amplified gene for epidermal growth factor receptor (EGFR) by the Southern hybridization technique using its complimentary DNA, pE7 instert, as a probe. Then, it was revealed that HOC 512 had an obviously amplified gene for EGFR when compared with that of normal human gingival cells. These results suggest that these cell lines are useful to investigate the biological features of oral cancer.
特定非営利活動法人日本口腔科学会の論文