Possibility on Appearance of Osteoclasts in Bone Marrow Cell Cultures. 2. Functional Characterization of Osteoclast-like Cells in Bone Marrow Cell Cultures.:2. Functional Characterization of Osteoclast-like Cells in Bone Marrow Cell Cultures

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Multinucleated giant cells not observed in the original medium, which were containted in bone marrow caltures, could be classified into 2 morphologically different types. One discoid and lobulated cell form (with 2 to 10 small, round nuclei per cell, enzymatically positive for β-glucuronidase, tartaric acid resistant and acid phosphatase positive) with undulating ruffling activity in the periphery was formed by cytoplasmic fusion. PTH increased the number of multinucleated cells and the number of nuclei per cell. Thus, these giant cells resembled osteoclasts very closely. Osteoclasts appeared in the culture medium when PTH and fetal guinea pig skulls were added simultaneously. These findings suggested that these multinucleated giant cells could transform into osteoclasts.
特定非営利活動法人日本口腔科学会の論文