A study on the optimal temperature for normal human cell culture derived from gingiva and salivary gland.

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Abstract: Effects of temperature on normal human cell culture of gingiva and salivary gland were examined at 27, 30, 33, 36, 39 and 42°C. Salivary gland epithelial cells grew well at 33°C, and gingival epithelial cells at 36°C. Fibroblastic cells derived from gingiva or salivary gland grew well at 36°C. At 30 or 33°C, epithelial cells maintained their sheet-like structure for weeks, inhibiting fibroblastic overgrowth. By shifting culture temperature to suboptimal range (27, 30 and 42°C), cell shape changed to round or flat types. Cultured cells were detached from substrate and suspended at 42°C, whereas they continued slow growth at 27, 30°C. These results show that optimal temperature in cell culture should be exactly determined according to the various cell types, especially in epithelial cell cultures.
特定非営利活動法人日本口腔科学会の論文