Development of an Enzyme-Linked Immunosorbent Assay(ELISA) for Detection of IgM and IgG Antibodies to Lipopolysaccharide of Escherichia coli O157.
スポンサーリンク
概要
- 論文の詳細を見る
We developed the enzyme-linked immunosorbent assay (ELISA) for detection of serum IgM and IgG antibodies to lipopolysaccharide (LPS) of <I>Escherichia coli</I> O157: H7 (VT1+, VT2+) isolated from a clinical sample. We tested the patient's sample with hemolytic uremic syndrome (HUS) and hemorrhagic colitis (HC). And we evaluated the value of sample OD to cut off control OD as the Index. The CVs obtained in precision studies were below 10% for intra-and inter-assay, respectively. The cut off index value of infants and children (0to5years) was 0.60 for IgM and 0.95 for IgG. Moreover we investigated the specificity to <I>E. coli</I> O157from other <I>E. coli</I> serotypes in this method. From these results, O27 serotype was crossreacted in this assay, but other serotypes were not reacted. In the patients with HUS and HC, some cases were already positive for IgM at 3days, but for IgG8days were needed for it to become positive. We concluded that this ELISA for IgM and IgG for <I>E. coli</I> O157 LPS have a useful performance, and might be the useful tool for rapid diagnosis for <I>E. coli</I> O157 infection.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
- Simple Method for Typing Human Immunodeficiency Virus Type 1 (B, E) by PCR and Chronological Change of Distribution ofSubtype (B to E) in Japan.
- Kinetic Analysis of Anti-HIV Titer in Early Stage of HIV Infection. A New Testing Strategy to Differentiate Early HIV Infection Individuals from False Positive Cases.:A New Testing Strategy to Differentiate Early HIV Infection Individuals from False Posit
- HIV-1 (Human Immunodeficiency Virus Type 1) Mutant Strain Associated with Zidovudine Resistance Detected from a Japanese Patient with Primary HIV-1 Infection.
- Epidemiological and Bacteriological Study on Gonococcal Infections.
- A Case of Trichosporon beigelii Peritonitis in CAPD.